Representative renal histology and quantitation of glomerulosclerosis and crescent formation. (a–d) Renal histology on day 21 (PAS staining). a and b, wild-type mice. c and d, Gas6–/– mice. Magnification: top panels, ×100; bottom panels, ×400. (e) Quantitative assessment of glomerulosclerosis and crescent formation in wild-type and Gas6–/– mice. Solid bars represent Gas6–/– mice, while open bars represent wild-type mice. Each group contained eight mice, and 40 glomeruli per mouse were evaluated in a blinded fashion. *P < 0.05. **P < 0.01.