Whole-genome amplification using Φ29 DNA polymerase

NP Burtt - Cold Spring Harbor Protocols, 2011 - cshprotocols.cshlp.org
NP Burtt
Cold Spring Harbor Protocols, 2011cshprotocols.cshlp.org
METHOD The start time of the reaction depends on the equipment used to carry out the
reaction. If a thermal cycler is used, program the robot to cool down to 4 C. The reaction
mixture can be kept at this temperature for hours. If an incubator is used, start the reaction in
the afternoon, so that the incubation will finish in the morning.
METHOD
The start time of the reaction depends on the equipment used to carry out the reaction. If a thermal cycler is used, program the robot to cool down to 4 C. The reaction mixture can be kept at this temperature for hours. If an incubator is used, start the reaction in the afternoon, so that the incubation will finish in the morning.
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