[HTML][HTML] Structure of Rpn10 and its interactions with polyubiquitin chains and the proteasome subunit Rpn12
C Riedinger, J Boehringer, JF Trempe, ED Lowe… - Journal of biological …, 2010 - ASBMB
Schizosaccharomyces pombe Rpn10 (SpRpn10) is a proteasomal ubiquitin (Ub) receptor
located within the 19 S regulatory particle where it binds to subunits of both the base and lid
subparticles. We have solved the structure of full-length SpRpn10 by determining the crystal
structure of the von Willebrand factor type A domain and characterizing the full-length
protein by NMR. We demonstrate that the single Ub-interacting motif (UIM) of SpRpn10
forms a 1: 1 complex with Lys 48-linked diUb, which it binds selectively over monoUb and …
located within the 19 S regulatory particle where it binds to subunits of both the base and lid
subparticles. We have solved the structure of full-length SpRpn10 by determining the crystal
structure of the von Willebrand factor type A domain and characterizing the full-length
protein by NMR. We demonstrate that the single Ub-interacting motif (UIM) of SpRpn10
forms a 1: 1 complex with Lys 48-linked diUb, which it binds selectively over monoUb and …