The use of reporter systems to analyze DNA double-strand break (DSB) repairs, based on
the enhanced green fluorescent protein (EGFP) and meganuclease such as I-Sce I, is
usually carried out with cell lines. In this study, we developed three visual-plus quantitative
assay systems for homologous recombination (HR), non-homologous end joining (NHEJ)
and single-strand annealing (SSA) DSB repair pathways at the organismal level in zebrafish
embryos. To initiate DNA DSB repair, we used two I-Sce I recognition sites in opposite …