The role of CD32 during HIV-1 infection
LN Bertagnolli, JA White, FR Simonetti, SA Beg, J Lai… - Nature, 2018 - nature.com
LN Bertagnolli, JA White, FR Simonetti, SA Beg, J Lai, C Tomescu, AJ Murray, AAR Antar…
Nature, 2018•nature.comMethods Participant recruitment and informed consent were performed under Institutional
Review Board (IRB)-approved protocols at the US National Institutes of Health (NIH). For
FACS, whole PBMCs were stained with monoclonal antibodies matching those used by
Descours et al. 1 (see Supplementary Methods) and sorted on a BD FACSAria. To evaluate
purity, a portion of each population was re-analysed on the flow cytometer after sorting. Virus
DNA copies in sorted cells were enumerated by fluorescence-assisted clonal amplification …
Review Board (IRB)-approved protocols at the US National Institutes of Health (NIH). For
FACS, whole PBMCs were stained with monoclonal antibodies matching those used by
Descours et al. 1 (see Supplementary Methods) and sorted on a BD FACSAria. To evaluate
purity, a portion of each population was re-analysed on the flow cytometer after sorting. Virus
DNA copies in sorted cells were enumerated by fluorescence-assisted clonal amplification …
Methods
Participant recruitment and informed consent were performed under Institutional Review Board (IRB)-approved protocols at the US National Institutes of Health (NIH). For FACS, whole PBMCs were stained with monoclonal antibodies matching those used by Descours et al. 1 (see Supplementary Methods) and sorted on a BD FACSAria. To evaluate purity, a portion of each population was re-analysed on the flow cytometer after sorting. Virus DNA copies in sorted cells were enumerated by fluorescence-assisted clonal amplification 2. DNA recovery was quantified by albumin (ALB) quantitative PCR. Because the FUN-2 monoclonal antibody used
nature.com