Composition of rat milk from day 5 to 20 of lactation and milk intake of lean and preobese Zucker pups
VY Godbole, ML Grundleger, TA Pasquine… - The Journal of …, 1981 - Elsevier
Milk composition and milk intake were measured in lean and preobese Zucker rat pups.
Lactating dams were injected with 3 H 2 O, which equilibrated with plasma water within 30
minutes. A constant specific activity of 3 H was maintained by adding 3 H 2 O to drinking
water. Blood samples of 5 µl were obtained from 10, 15 and 20-day-old suckling pups every
4 hours for a 24-hour period and the accumulation of tritium was used to calculate pups' milk
intake. No diurnal variation in milk intake of pups was observed. Obese pups were identified …
Lactating dams were injected with 3 H 2 O, which equilibrated with plasma water within 30
minutes. A constant specific activity of 3 H was maintained by adding 3 H 2 O to drinking
water. Blood samples of 5 µl were obtained from 10, 15 and 20-day-old suckling pups every
4 hours for a 24-hour period and the accumulation of tritium was used to calculate pups' milk
intake. No diurnal variation in milk intake of pups was observed. Obese pups were identified …