[PDF][PDF] RNA sequencing of single human islet cells reveals type 2 diabetes genes

Y Xin, J Kim, H Okamoto, M Ni, Y Wei, C Adler… - Cell metabolism, 2016 - cell.com
Y Xin, J Kim, H Okamoto, M Ni, Y Wei, C Adler, AJ Murphy, GD Yancopoulos, C Lin…
Cell metabolism, 2016cell.com
Pancreatic islet cells are critical for maintaining normal blood glucose levels, and their
malfunction underlies diabetes development and progression. We used single-cell RNA
sequencing to determine the transcriptomes of 1,492 human pancreatic α, β, δ, and PP cells
from non-diabetic and type 2 diabetes organ donors. We identified cell-type-specific genes
and pathways as well as 245 genes with disturbed expression in type 2 diabetes.
Importantly, 92% of the genes have not previously been associated with islet cell function or …
Summary
Pancreatic islet cells are critical for maintaining normal blood glucose levels, and their malfunction underlies diabetes development and progression. We used single-cell RNA sequencing to determine the transcriptomes of 1,492 human pancreatic α, β, δ, and PP cells from non-diabetic and type 2 diabetes organ donors. We identified cell-type-specific genes and pathways as well as 245 genes with disturbed expression in type 2 diabetes. Importantly, 92% of the genes have not previously been associated with islet cell function or growth. Comparison of gene profiles in mouse and human α and β cells revealed species-specific expression. All data are available for online browsing and download and will hopefully serve as a resource for the islet research community.
cell.com