Locally injected Infliximab ameliorates murine DSS colitis: differences in serum and intestinal levels of drug between healthy and colitic mice

LR Lopetuso, V Petito, V Cufino, V Arena… - Digestive and liver …, 2013 - Elsevier
Background Infliximab is effective in human and murine IBD, but its pharmacodynamic is still
poorly known. The aim of this study was to assess the affinity of infliximab to murine TNF-α,
its role in murine colitis when administered intra-rectally and its levels in the blood, gut
mucosa and stool of healthy and sick mice. Methods An ELISA kit was built in order to
assess the affinity of infliximab to human or murine-TNF-α. Human IgG were used as
controls. DSS model of colitis on C57BL/6 mice was used to assess clinical efficacy of …