Stimulation of adipogenesis in fibroblasts by PPARγ2, a lipid-activated transcription factor

P Tontonoz, E Hu, BM Spiegelman - Cell, 1994 - cell.com
Cell, 1994cell.com
Peroxisome proliferator-activated receptor 72 (PPARy2) is an adipocyte-specific nuclear
hormone receptor that has recently been identified as a key regulator of two fat cell
enhancers. Transcriptional activation by PPARy2 is potentiated by a variety of lipids and
lipid-like compounds, including naturally occurring polyunsaturated fatty acids. We
demonstrate here that retroviral expression of PPARyP stimulates adipose differentiation of
cultured fibroblasts. PPAR activators promote the differentiation of PPARy%-expressing cells …
Summary
Peroxisome proliferator-activated receptor 72 (PPARy2) is an adipocyte-specific nuclear hormone receptor that has recently been identified as a key regulator of two fat cell enhancers. Transcriptional activation by PPARy2 is potentiated by a variety of lipids and lipid-like compounds, including naturally occurring polyunsaturated fatty acids. We demonstrate here that retroviral expression of PPARyP stimulates adipose differentiation of cultured fibroblasts. PPAR activators promote the differentiation of PPARy%-expressing cells in a dose-dependent manner. CIEBPcc, a second transcription factor induced during adipocyte differentiation, can cooperate with PPARy2 to stimulate the adipocyte program dramatically. Our results suggest that the physiologic role of PPARy2 is to regulate development of the adipose lineage in response to endogenous lipid activators and that this factor may serve to link the process of adipocyte differentiation to systemic lipid metabolism.
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