Pretreatment of polystyrene microplate wells with certain doses of UV light enhances their
capacity for binding to single-stranded DNA, double stranded DNA and various synthetic
polynucleotides. The use of UV-irradiated plates to immobilize nucleic acid antigens
provides a simple, rapid, and specific ELISA for measuring anti-nucleic acid antibodies. The
assay is at least as sensitive as the more complex method of precoating plates with poly (L-
lysine). It is useful for detection of anti-DNA antibodies in sera of systemic lupus …