There is compelling evidence that aminoglycoside (AG) antibiotics can induce the
mammalian ribosome to suppress disease-causing nonsense mutations and partially
restore the expression of functional proteins. However, prolonged AG treatment can cause
detrimental side effects in patients, including most prominently, ototoxicity. Recent
mechanistic discussions have considered the relative contributions of mitochondrial and
cytoplasmic protein synthesis inhibition to AG-induced ototoxicity. We show that AGs inhibit …