Calcium, free radicals, and excitotoxic neuronal death in primary cell culture
Publisher Summary Elucidation of cellular and molecular mechanisms of excitotoxic
neuronal injury have been facilitated by the development of several cell biological
technologies—namely,(1) neural cell culture,(2) quantitative assays of neuronal injury and
death,(3) fluorescence imaging of intracellular free calcium levels ([Ca 2+] i),(4) evaluation of
mitochondrial function,(5) quantification of reactive oxygen species,(6) assessment of
cytoskeletal alterations elicited by excitotoxic/metabolic insults, and (7) detection of “stress …
neuronal injury have been facilitated by the development of several cell biological
technologies—namely,(1) neural cell culture,(2) quantitative assays of neuronal injury and
death,(3) fluorescence imaging of intracellular free calcium levels ([Ca 2+] i),(4) evaluation of
mitochondrial function,(5) quantification of reactive oxygen species,(6) assessment of
cytoskeletal alterations elicited by excitotoxic/metabolic insults, and (7) detection of “stress …
