Mitochondrial dysfunction is associated with many human diseases. Mitochondrial damage
is exacerbated by inadequate protein quality control and often further contributes to
pathogenesis. The maintenance of mitochondrial functions requires a delicate balance of
continuous protein synthesis and degradation, ie protein turnover. To understand
mitochondrial protein dynamics in vivo, we designed a metabolic heavy water (2 H 2 O)
labeling strategy customized to examine individual protein turnover in the mitochondria in a …