Mechanisms of abruption-induced premature rupture of the fetal membranes: Thrombin enhanced decidual matrix metalloproteinase-3 (stromelysin-1) expression
AP Mackenzie, F Schatz, G Krikun, EF Funai… - American journal of …, 2004 - Elsevier
OBJECTIVE: The aim of the study was to evaluate thrombin and progestin effects on matrix
metalloproteinase-3 expression in term decidual cells as a mechanism of abruption-related
preterm delivery. STUDY DESIGN: Decidual cells were isolated by standard techniques,
purified to homogeneity, grown to confluence, and passaged. Cultures were primed with 10−
8 M estradiol or estradiol plus 10− 7 progestin and then incubated in a defined medium with
corresponding steroid (s) plus or minus thrombin or the protease-activated thrombin receptor …
metalloproteinase-3 expression in term decidual cells as a mechanism of abruption-related
preterm delivery. STUDY DESIGN: Decidual cells were isolated by standard techniques,
purified to homogeneity, grown to confluence, and passaged. Cultures were primed with 10−
8 M estradiol or estradiol plus 10− 7 progestin and then incubated in a defined medium with
corresponding steroid (s) plus or minus thrombin or the protease-activated thrombin receptor …