From lysosomes to the plasma membrane: localization of vacuolar type H+-ATPase with the a3 isoform during osteoclast differentiation
T Toyomura, Y Murata, A Yamamoto, T Oka… - Journal of Biological …, 2003 - ASBMB
Osteoclasts generate a massive acid flux to mobilize bone calcium. Local extracellular
acidification is carried out by vacuolar type H+-ATPase (V-ATPase) localized in the plasma
membrane. We have shown that a3, one of the four subunit a isoforms (a1, a2, a3, and a4),
is a component of the plasma membrane V-ATPase (Toyomura, T., Oka, T., Yamaguchi, C.,
Wada, Y., and Futai, M.(2000) J. Biol. Chem. 275, 8760–8765). To establish the unique
localization of V-ATPase, we have used a murine macrophage cell line, RAW 264.7, that can …
acidification is carried out by vacuolar type H+-ATPase (V-ATPase) localized in the plasma
membrane. We have shown that a3, one of the four subunit a isoforms (a1, a2, a3, and a4),
is a component of the plasma membrane V-ATPase (Toyomura, T., Oka, T., Yamaguchi, C.,
Wada, Y., and Futai, M.(2000) J. Biol. Chem. 275, 8760–8765). To establish the unique
localization of V-ATPase, we have used a murine macrophage cell line, RAW 264.7, that can …
