TRPC1 is required for functional store-operated Ca2+ channels: role of acidic amino acid residues in the S5-S6 region
X Liu, BB Singh, IS Ambudkar - Journal of Biological Chemistry, 2003 - ASBMB
The exact role of TRPC1 in store-operated calcium influx channel (SOCC) function is not
known. We have examined the effect of overexpression of full-length TRPC1, depletion of
endogenous TRPC1, and expression of TRPC1 in which the proposed pore region (S5-S6,
amino acids (aa) 557–620) was deleted or modified by site-directed mutagenesis on
thapsigargin-and carbachol-stimulated SOCC activity in HSG cells. TRPC1 overexpression
induced channel activity that was indistinguishable from the endogenous SOCC activity …
known. We have examined the effect of overexpression of full-length TRPC1, depletion of
endogenous TRPC1, and expression of TRPC1 in which the proposed pore region (S5-S6,
amino acids (aa) 557–620) was deleted or modified by site-directed mutagenesis on
thapsigargin-and carbachol-stimulated SOCC activity in HSG cells. TRPC1 overexpression
induced channel activity that was indistinguishable from the endogenous SOCC activity …
