Adenine nucleotide metabolism by isolated kidney tubules during oxygen deprivation
JM Weinberg - Biochemical medicine and metabolic biology, 1988 - Elsevier
Suspensions enriched in isolated rabbit proximal tubules were subjected to varying degrees
of oxygen deprivation-induced injury by incubating them under hypoxic conditions at pH 7.4
or pH 6.6 or under high density pelleted conditions and adenine nucleotide degradation was
characterized. The major metabolite was hypoxanthine. Its levels increased with the extent
of irreversible injury. It was not further degraded or salvaged. Recovery of cell ATP during
reoxygenation was predominantly from the remaining cell nucleotides. Allopurinol did not …
of oxygen deprivation-induced injury by incubating them under hypoxic conditions at pH 7.4
or pH 6.6 or under high density pelleted conditions and adenine nucleotide degradation was
characterized. The major metabolite was hypoxanthine. Its levels increased with the extent
of irreversible injury. It was not further degraded or salvaged. Recovery of cell ATP during
reoxygenation was predominantly from the remaining cell nucleotides. Allopurinol did not …
