[2] Long-term storage of mitochondria to preserve energy-linked functions

S Fleischer - Methods in Enzymology, 1979 - Elsevier
Publisher Summary This chapter describes a procedure for the storage of rat liver
mitochondria to preserve mitochondrial functions for prolonged periods (months). The
availability of many tubes of mitochondria from one large-scale preparation emancipates the
investigator from the daily tedium of isolation. Such a stock eliminates individual differences
in animals and inadvertent changes in the isolation procedure. The chapter discusses three
parameters of mitochondrial function, each of which has been preserved by the method of …