Considerable disparities in the reported levels of basal human nitrite and S-nitrosothiols
(RSNO) in blood have brought methods of quantifying these nitric oxide (NO) metabolites to
the forefront of NO biology. Ozone-based chemiluminescence is commonly used and is a
robust method for measuring these species when combined with proper reductive chemistry.
The goal of this article is to review existing methodologies for the measurement of nitrite and
RSNO by reductive chemiluminescence. Specifically, we discuss in detail the measurement …