Purification of hematopoietic stem cells using the side population
KK Lin, MA Goodell - Methods in enzymology, 2006 - Elsevier
Hematopoietic stem cells (HSCs) primarily reside in bone marrow, are defined by their ability
to maintain blood homeostasis, and replenish themselves through self‐renewal. Although
HSC purification schemes vary from laboratory to laboratory, the resulting cell populations
are similar, if not the same. This chapter will discuss different enrichment methods for HSCs
and provide a detailed protocol for staining HSC with Hoechst 33342 for the side population
(SP).
to maintain blood homeostasis, and replenish themselves through self‐renewal. Although
HSC purification schemes vary from laboratory to laboratory, the resulting cell populations
are similar, if not the same. This chapter will discuss different enrichment methods for HSCs
and provide a detailed protocol for staining HSC with Hoechst 33342 for the side population
(SP).
