The stimulatory effect of vasoactive intestinal peptide (VIP) and analogues on [Ca2+] ihas
been investigated in chinese hamster ovary (CHO) cells stably transfected with the reporter
gene aequorin, and expressing either the human VPAC1or VPAC2receptor in absence or in
presence of the Gα16. In cells that were not transfected with Gα16 and expressed a similar
density of receptors, the VIP induced [Ca2+] iincrease was higher in VPAC1than in
VPAC2receptor expressing cells. In aequorin/Gα16 cotransfected cells, the VIP-induced …