Murine IgG1 and IgE memory B cells
H Takahama, Z Ovary, S Furusawa - Cellular immunology, 1994 - Elsevier
Abstract Anti-dinitrophenyl IgG1 and IgE antibody production were examined in vitro.
Splenic B cells from mice immunized in vivo at various intervals with dinitrophenylated
proteins were cultured in vitro with splenic T cells and splenic antigen-presenting cells from
mice immunized previously (2 weeks) with keyhole limpet hemocyanin. Anti-dinitrophenyl
IgG1 and IgE antibodies were determined in the supernatants by ELISA. Without T cells and
antigen-presenting cells or without antigen, no anti-dinitrophenyl antibodies were detected …
Splenic B cells from mice immunized in vivo at various intervals with dinitrophenylated
proteins were cultured in vitro with splenic T cells and splenic antigen-presenting cells from
mice immunized previously (2 weeks) with keyhole limpet hemocyanin. Anti-dinitrophenyl
IgG1 and IgE antibodies were determined in the supernatants by ELISA. Without T cells and
antigen-presenting cells or without antigen, no anti-dinitrophenyl antibodies were detected …