Immunoglobulins (Igs) on the surface of B lymphocytes are isotype-specific immunological markers of the B-cell subsets expressing them. Since these membrane-bound Igs (mIgs) are antigen receptors, their interaction with antibodies could be explored for modulating the activity of specific B-cell subsets. Targeting mIgs by antibodies in vivo, however, has not been feasible because of the presence of Igs in the circulation and the frequent association of Igs with various cell types via Fc receptors. To circumvent these problems, we proposed that the extracellular portions of the membrane-anchoring segments of the heavy chains of mIgs, referred to as “mIg isotype-specific” or “migis” peptides, may provide the antigenic sites for the isotype-specific targeting of B cells in vivo. Here we describe the exemplary development of monoclonal antibodies (mAbs) recognizing this unique epitope of mIgE.