End-joining of blunt DNA double-strand breaks in mammalian fibroblasts is precise and requires DNA-PK and XRCC4
D van Heemst, L Brugmans, NS Verkaik, DC van Gent - DNA repair, 2004 - Elsevier
DNA double-strand break repair by non-homologous end-joining (NHEJ) is generally
considered to be an imprecise repair pathway. In order to study repair of a blunt, 5′
phosphorylated break in the DNA of mammalian fibroblasts, we used the E. coli cut-and-
paste type transposon Tn5. We found that the Tn5 transposase can mediate transposon
excision in Chinese hamster cell lines. Interestingly, a blunt 5′ phosphorylated break could
efficiently be repaired without loss of nucleotides in wild type fibroblasts. Catalytic subunit of …
considered to be an imprecise repair pathway. In order to study repair of a blunt, 5′
phosphorylated break in the DNA of mammalian fibroblasts, we used the E. coli cut-and-
paste type transposon Tn5. We found that the Tn5 transposase can mediate transposon
excision in Chinese hamster cell lines. Interestingly, a blunt 5′ phosphorylated break could
efficiently be repaired without loss of nucleotides in wild type fibroblasts. Catalytic subunit of …
