Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line
P Masiakowski, R Breathnach, J Bloch… - Nucleic acids …, 1982 - academic.oup.com
P Masiakowski, R Breathnach, J Bloch, F Gannon, A Krust, P Chambon
Nucleic acids research, 1982•academic.oup.comAbstract cDNA clones corresponding to a mRNA whose level is rapidly increased by
addition of oestradiol to the culture medium have been isolated by differential screening of a
cDNA library from the breast cancer cell line MCF-7, which contains oestrogen receptors.
Such clones will be useful in studies of the DNA sequences required for hormonal induction
and to determine whether expression of the corresponding gene is in any way related to the
cancerous state. We have also obtained a cDNA clone for a messenger whose level is …
addition of oestradiol to the culture medium have been isolated by differential screening of a
cDNA library from the breast cancer cell line MCF-7, which contains oestrogen receptors.
Such clones will be useful in studies of the DNA sequences required for hormonal induction
and to determine whether expression of the corresponding gene is in any way related to the
cancerous state. We have also obtained a cDNA clone for a messenger whose level is …
Abstract
cDNA clones corresponding to a mRNA whose level is rapidly increased by addition of oestradiol to the culture medium have been isolated by differential screening of a cDNA library from the breast cancer cell line MCF-7, which contains oestrogen receptors. Such clones will be useful in studies of the DNA sequences required for hormonal induction and to determine whether expression of the corresponding gene is in any way related to the cancerous state. We have also obtained a cDNA clone for a messenger whose level is apparently decreased by steroid hormones
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