An improved method for the determination of catecholamines in biological fluids, by
reversed-phase high-performance liquid chromatography (HPLC) with fluorimetric detection
is presented. The pH titration previously employed in the alumina extraction was abandoned
in favour of the use of a molar excess of pH 8.5 Tris—HCl buffer. A novel lyophilisation step
serves to concentrate the catechols and by reconstituting in mobile phase, chromatography
disturbances are minimised. The addition of 2 mM octanesulphonic acid to a citrate …