In vitro platelet release by rat megakaryocytes: effect of metabolic inhibitors and cytoskeletal disrupting agents.
PJ Handagama, BF Feldman, NC Jain… - American journal of …, 1987 - europepmc.org
PJ Handagama, BF Feldman, NC Jain, TB Farver, CS Kono
American journal of veterinary research, 1987•europepmc.orgDevelopment of an in vitro visual assay facilitated the study of large numbers of
megakaryocytes undergoing proplatelet formation in short-term cultures. Approximately 9%
of megakaryocytes formed platelets during a 24-hour period. In the presence of an inhibitor
of anaerobic glycolysis (NaF), proplatelet formation was inhibited, whereas inhibitors of
respiration (NaCN) did not significantly (P greater than 0.05) decrease proplatelet formation.
Presence of the microtubule-disrupting agents colchicine and vincristine sulfate in culture …
megakaryocytes undergoing proplatelet formation in short-term cultures. Approximately 9%
of megakaryocytes formed platelets during a 24-hour period. In the presence of an inhibitor
of anaerobic glycolysis (NaF), proplatelet formation was inhibited, whereas inhibitors of
respiration (NaCN) did not significantly (P greater than 0.05) decrease proplatelet formation.
Presence of the microtubule-disrupting agents colchicine and vincristine sulfate in culture …
Development of an in vitro visual assay facilitated the study of large numbers of megakaryocytes undergoing proplatelet formation in short-term cultures. Approximately 9% of megakaryocytes formed platelets during a 24-hour period. In the presence of an inhibitor of anaerobic glycolysis (NaF), proplatelet formation was inhibited, whereas inhibitors of respiration (NaCN) did not significantly (P greater than 0.05) decrease proplatelet formation. Presence of the microtubule-disrupting agents colchicine and vincristine sulfate in culture medium inhibited proplatelet formation, whereas the microfilament-disrupting agent cytochalasin B had a less pronounced inhibition.
europepmc.org
