Improved silver staining protocols for high sensitivity protein identification using matrix‐assisted laser desorption/ionization‐time of flight analysis
E Mortz, TN Krogh, H Vorum… - Proteomics: International …, 2001 - Wiley Online Library
E Mortz, TN Krogh, H Vorum, A Görg
Proteomics: International Edition, 2001•Wiley Online LibraryIn proteomics it is essential to be able to detect proteins separated by gel electrophoresis at
high sensitivity. Silver staining is currently the most popular method. Here we present silver
staining protocols that are optimized for staining sensitivity, peptide recovery and
compatibility with digestion and mass spectrometry.
high sensitivity. Silver staining is currently the most popular method. Here we present silver
staining protocols that are optimized for staining sensitivity, peptide recovery and
compatibility with digestion and mass spectrometry.
Abstract
In proteomics it is essential to be able to detect proteins separated by gel electrophoresis at high sensitivity. Silver staining is currently the most popular method. Here we present silver staining protocols that are optimized for staining sensitivity, peptide recovery and compatibility with digestion and mass spectrometry.
Wiley Online Library