Induction of bone morphogenetic protein-2 by interleukin-1 in human fibroblasts
MJ Fowler Jr, MS Neff, RC Borghaei, EA Pease… - Biochemical and …, 1998 - Elsevier
MJ Fowler Jr, MS Neff, RC Borghaei, EA Pease, E Mochan, RD Thornton
Biochemical and biophysical research communications, 1998•ElsevierRheumatoid arthritis and periodontitis are chronic inflammatory diseases associated with
tissue destruction that is mediated in part by elevated levels of cytokines (eg, interleukin-1
and tumor necrosis factor). Differential screening of a human synovial fibroblast cDNA library
for interleukin-1 induced genes revealed a clone identical to the gene encoding human
bone morphogenetic protein-2. Northern blot analysis of human synovial fibroblast mRNA
confirmed up-regulation of bone morphogenetic protein-2 in the presence of interleukin-1 …
tissue destruction that is mediated in part by elevated levels of cytokines (eg, interleukin-1
and tumor necrosis factor). Differential screening of a human synovial fibroblast cDNA library
for interleukin-1 induced genes revealed a clone identical to the gene encoding human
bone morphogenetic protein-2. Northern blot analysis of human synovial fibroblast mRNA
confirmed up-regulation of bone morphogenetic protein-2 in the presence of interleukin-1 …
Rheumatoid arthritis and periodontitis are chronic inflammatory diseases associated with tissue destruction that is mediated in part by elevated levels of cytokines (e.g., interleukin-1 and tumor necrosis factor). Differential screening of a human synovial fibroblast cDNA library for interleukin-1 induced genes revealed a clone identical to the gene encoding human bone morphogenetic protein-2. Northern blot analysis of human synovial fibroblast mRNA confirmed up-regulation of bone morphogenetic protein-2 in the presence of interleukin-1. Utilizing a specific antibody, levels of bone morphogenetic protein-2 protein in conditioned medium from synovial fibroblasts were also up-regulated in the presence of interleukin-1. This is the first report of the production of bone morphogenetic protein-2 by synovial fibroblasts, and the first report of its up-regulation in response to interleukin-1. However, interleukin-1 did not induce bone morphogenetic protein-2 mRNA in human gingival fibroblasts.
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