Characterization of the hemin-sensitive eukaryotic initiation factor 2α kinase from mouse nonerythroid cells
JJ Berlanga, S Herrero, C de Haro - Journal of Biological Chemistry, 1998 - ASBMB
The heme-regulated eukaryotic initiation factor 2α (eIF2α) kinase (heme-regulated inhibitor
(HRI)) is activated by heme deficiency in reticulocytes and plays an important role in
translational control in these cells. Previously, HRI was cloned from rabbit reticulocytes and
rat brain, but a heme-regulated eIF2α kinase activity has only been purified from erythroid
cells. In this study, we report the purification of a heme-sensitive eIF2α kinase activity from
both mouse liver and NIH 3T3 cell extracts. Furthermore, we have cloned and characterized …
(HRI)) is activated by heme deficiency in reticulocytes and plays an important role in
translational control in these cells. Previously, HRI was cloned from rabbit reticulocytes and
rat brain, but a heme-regulated eIF2α kinase activity has only been purified from erythroid
cells. In this study, we report the purification of a heme-sensitive eIF2α kinase activity from
both mouse liver and NIH 3T3 cell extracts. Furthermore, we have cloned and characterized …
