Differences in proliferation of the hematopoietic cell line TF-1 and cytokine production by peripheral blood leukocytes induced by 2 naturally occurring forms of human …
A Schweiger, D Stern, IC Lohman, M Baldini… - Journal of allergy and …, 2001 - Elsevier
Background: A naturally occurring polymorphism in the coding region of the human IL3 gene
leads to a change in amino acid residue 8 from proline to serine. Objective: We sought to
determine whether the 2 different forms of IL-3 varied in function. These different forms are
available as recombinant proteins (recombinant human IL-3/proline 8 [rhIL-3/P8] and
recombinant human IL-3/serine 8 [rhIL-3/S8]). Methods: The erythroleukemic cell line TF-1
was incubated with varying concentrations of rhIL-3/P8 or rhIL-3/S8 to determine the …
leads to a change in amino acid residue 8 from proline to serine. Objective: We sought to
determine whether the 2 different forms of IL-3 varied in function. These different forms are
available as recombinant proteins (recombinant human IL-3/proline 8 [rhIL-3/P8] and
recombinant human IL-3/serine 8 [rhIL-3/S8]). Methods: The erythroleukemic cell line TF-1
was incubated with varying concentrations of rhIL-3/P8 or rhIL-3/S8 to determine the …