Publisher Summary This chapter observes the regulation of calcineurin by oxidative stress.
The protection of calcineurin against inactivation by superoxide dismutase and its
reactivation by ascorbate, led to propose that inactivation was the result of oxidation of the
iron cofactor. To identify the oxidation state of iron in the native enzyme, the purification
procedure had to be modified to prevent the loss of the natural cofactors. Different assay
conditions were used throughout the purification to monitor both the depletion of metal …