[HTML][HTML] Inducible nitric-oxide synthase generates superoxide from the reductase domain
Y Xia, LJ Roman, BSS Masters, JL Zweier - Journal of Biological Chemistry, 1998 - ASBMB
In the absence of l-arginine, the heme center of the oxygenase domain of neuronal nitric-
oxide synthase reduces molecular oxygen to superoxide (O⨪ 2). Our recent work has
provided evidence that inducible NOS (iNOS) may also catalyze O⨪ 2 formation in
macrophages. However, there has been a lack of direct evidence of superoxide generation
from the purified iNOS, and it was previously hypothesized that significant O⨪ 2 production
does not occur. Moreover, the mechanism and enzyme site responsible for O⨪ 2 generation …
oxide synthase reduces molecular oxygen to superoxide (O⨪ 2). Our recent work has
provided evidence that inducible NOS (iNOS) may also catalyze O⨪ 2 formation in
macrophages. However, there has been a lack of direct evidence of superoxide generation
from the purified iNOS, and it was previously hypothesized that significant O⨪ 2 production
does not occur. Moreover, the mechanism and enzyme site responsible for O⨪ 2 generation …