Transduction of Human CD34+ Cells That Mediate Long-Term Engraftment of NOD/SCID Mice by HIV Vectors
H Miyoshi, KA Smith, DE Mosier, IM Verma, BE Torbett - Science, 1999 - science.org
H Miyoshi, KA Smith, DE Mosier, IM Verma, BE Torbett
Science, 1999•science.orgEfficient gene transfer into human hematopoietic stem cells (HSCs) is an important goal in
the study of the hematopoietic system as well as for gene therapy of hematopoietic
disorders. A lentiviral vector based on the human immunodeficiency virus (HIV) was able to
transduce human CD34+ cells capable of stable, long-term reconstitution of nonobese
diabetic/severe combined immunodeficient (NOD/SCID) mice. High-efficiency transduction
occurred in the absence of cytokine stimulation and resulted in transgene expression in …
the study of the hematopoietic system as well as for gene therapy of hematopoietic
disorders. A lentiviral vector based on the human immunodeficiency virus (HIV) was able to
transduce human CD34+ cells capable of stable, long-term reconstitution of nonobese
diabetic/severe combined immunodeficient (NOD/SCID) mice. High-efficiency transduction
occurred in the absence of cytokine stimulation and resulted in transgene expression in …
Efficient gene transfer into human hematopoietic stem cells (HSCs) is an important goal in the study of the hematopoietic system as well as for gene therapy of hematopoietic disorders. A lentiviral vector based on the human immunodeficiency virus (HIV) was able to transduce human CD34+ cells capable of stable, long-term reconstitution of nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. High-efficiency transduction occurred in the absence of cytokine stimulation and resulted in transgene expression in multiple lineages of human hematopoietic cells for up to 22 weeks after transplantation.
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