[HTML][HTML] Receptor number and caveolar co-localization determine receptor coupling efficiency to adenylyl cyclase
Recent evidence suggests that many signaling molecules localize in microdomains of the
plasma membrane, particularly caveolae. In this study, overexpression of adenylyl cyclase
was used as a functional probe of G protein-coupled receptor (GPCR) compartmentation.
We found that three endogenous receptors in neonatal rat cardiomyocytes couple with
different levels of efficiency to the activation of adenylyl cyclase type 6 (AC6), which localizes
to caveolin-rich membrane fractions. Overexpression of AC6 enhanced the maximal cAMP …
plasma membrane, particularly caveolae. In this study, overexpression of adenylyl cyclase
was used as a functional probe of G protein-coupled receptor (GPCR) compartmentation.
We found that three endogenous receptors in neonatal rat cardiomyocytes couple with
different levels of efficiency to the activation of adenylyl cyclase type 6 (AC6), which localizes
to caveolin-rich membrane fractions. Overexpression of AC6 enhanced the maximal cAMP …
