Measurement of the testosterone binding parameters for both testosterone-estradiol binding globulin and albumin in individual serum samples
BC Nisula, JF Dunn - Steroids, 1979 - Elsevier
This report describes a solid phase method for the characterization of testosterone binding
to both albumin and testosterone-estradiol binding globulin (TeBG). TeBG is adsorbed from
serum samples onto a solid phase matrix of concanavalin A covalently linked to 4B
Sepharose. The binding of testosterone is then examined both in the presence and absence
of the endogenous serum albumin. Analysis of the resulting Scatchard plots permits
determination of the TeBG binding capacity, TeBG association constant and a parameter of …
to both albumin and testosterone-estradiol binding globulin (TeBG). TeBG is adsorbed from
serum samples onto a solid phase matrix of concanavalin A covalently linked to 4B
Sepharose. The binding of testosterone is then examined both in the presence and absence
of the endogenous serum albumin. Analysis of the resulting Scatchard plots permits
determination of the TeBG binding capacity, TeBG association constant and a parameter of …
