Specific uncoupling of GRB2 from the met receptor: differential effects on transformation and motility
C Ponzetto, Z Zhen, E Audero, F Maina… - Journal of Biological …, 1996 - ASBMB
The biological effects of hepatocyte growth factor/scatter factor are mediated by
autophosphorylation of its receptor, the Met tyrosine kinase, on two carboxyl-terminal
tyrosines. These phosphotyrosines (Y 1349 VHVNATY 1356 VNV) are multifunctional
docking sites for several effectors. Grb2, the adaptor for the Ras guanyl-nucleotide
exchanger SOS, binds to Tyr 1356 in the YVV motif. By site-directed mutagenesis we either
abrogated or duplicated the Grb2 consensus, without interfering with the other effectors …
autophosphorylation of its receptor, the Met tyrosine kinase, on two carboxyl-terminal
tyrosines. These phosphotyrosines (Y 1349 VHVNATY 1356 VNV) are multifunctional
docking sites for several effectors. Grb2, the adaptor for the Ras guanyl-nucleotide
exchanger SOS, binds to Tyr 1356 in the YVV motif. By site-directed mutagenesis we either
abrogated or duplicated the Grb2 consensus, without interfering with the other effectors …
