[HTML][HTML] The expression of constitutively active isotypes of protein kinase C to investigate preconditioning
J Zhao, O Renner, L Wightman, PH Sugden… - Journal of Biological …, 1998 - ASBMB
The role of protein kinase C (PKC) in ischemic preconditioning remains controversial
because of difficulties with both its measurement and pharmacological manipulation. We
investigated preconditioning in isolated neonatal rat cardiocytes by expressing constitutively
active isotypes of PKC. Observations at differing durations of simulated ischemia suggested
β-galactosidase (β-gal) activity reflected viability within transfected myocytes.
Preconditioning with 90 min of ischemia significantly increased β-gal activity and myocyte …
because of difficulties with both its measurement and pharmacological manipulation. We
investigated preconditioning in isolated neonatal rat cardiocytes by expressing constitutively
active isotypes of PKC. Observations at differing durations of simulated ischemia suggested
β-galactosidase (β-gal) activity reflected viability within transfected myocytes.
Preconditioning with 90 min of ischemia significantly increased β-gal activity and myocyte …
